Optimising automation of a manual enzyme-linked immunosorbent assay
نویسندگان
چکیده
منابع مشابه
Optimising automation of a manual enzyme-linked immunosorbent assay
OBJECTIVE Enzyme-linked immunosorbent assays (ELISAs) are widely used to quantify immunoglobulin levels induced by infection or vaccination. Compared to conventional manual assays, automated ELISA systems offer more accurate and reproducible results, faster turnaround times and cost effectiveness due to the use of multianalyte reagents. DESIGN The VaccZyme™ Human Anti-Haemophilus influenzae t...
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An enzyme-linked immunosorbent assay for neopterin using penicillinase as marker enzyme is reported here by polyclonal antibodies against neopterin conjugated to bovine serum albumin which were raised in rabbits. Immunoglobulin fractions were purified and coated on wells of microtiter plates. A chain heterology was introduced in neopterin derivative and conjugated to penicillinase. The assa...
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An enzyme-linked immunosorbent assay was used to titrate antisera raised against live cultures of eight type species (biotypes) of Bacteroides with the EDTA-released outer-membrane complex from 29 characterized strains of Bacteroides species. With only minor exceptions, the strains investigated reacted to titre with the antisera raised against the homologous type species and not against the het...
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ژورنال
عنوان ژورنال: African Journal of Laboratory Medicine
سال: 2011
ISSN: 2225-2010,2225-2002
DOI: 10.4102/ajlm.v1i1.15